Journal: Science Progress

Article Title: Low-dose urapidil mitigates renal ischemia-reperfusion injury through matrix metalloproteinase-9 inhibition and anti-inflammatory effects

doi: 10.1177/00368504261438886

Figure Lengend Snippet: Comparison of MMP-9 IHC between the kidney samples from each group. Details of MMP-9 marking were observed with IHC. MMP-9, matrix metalloproteinase-9; IHC, immunohistochemistry; SH, sham group; CR, control group; I/R, ischemia-reperfusion injury; UP, urapidil.

Article Snippet: Following the completion of the blocking procedure, the samples were treated with primary antibodies, including eNOS (Boster Bio., catalog number: A01604-2, diluted at a ratio of 1:250), caspase 3 (Thermo Scıentıfıc, catalog number: RB-1197-P0, diluted at a ratio of 1:100), TNF-α (Proteintech, catalog number:60291-1-Ig, diluted at a ratio of 1:300), IL-1β (Bioss, catalog number:bs-6319R, diluted at a ratio of 1:150), IL-6 (ST John’s, catalog number:STJ1110424, diluted at a ratio of 1:100), and MMP-9 (Boster Bio, catalog number: PA2140-2, diluted at a ratio of 1:100).

Techniques: Comparison, Immunohistochemistry, Control

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Novel platelet-rich plasma/ hyaluronic acid lyophilized formulations for wound healing applications

doi: 10.3389/fbioe.2025.1619633

Figure Lengend Snippet: (A) RNA was extracted from hFB cells after 6 h of treatment, and qRT-PCR was performed for COL1A1 and ELS gene expressions. (B) COL1A1 and ELS protein expressions, after 24 h, using Western blotting in hFB cells. The level of each protein was normalized to GAPDH expression, which was used as the housekeeping internal control.

Article Snippet: Anti COL1A1 (110 kDa, Elabscience, Houston, TX, United States) and ELS (60 kDa, Elabscience) primary antibodies were used at 1:250 dilutions.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Control

Journal: Neoplasia (New York, N.Y.)

Article Title: Hypoxia Inducible Factors Modify Collagen I Fibers in MDA-MB-231 Triple Negative Breast Cancer Xenografts 1 2

doi: 10.1016/j.neo.2017.11.010

Figure Lengend Snippet: (A) Representative 1X and 20X images of COL1A1 immunostained tumor sections. (B) Quantification of strongly positive pixels normalized to the area of the tumor section. Values represent Mean ± S.E.M. * P ≤ .05. (C) Representative immunoblots showing COL1A1 levels in 231-EV, 231-1α, 231-2α, and 231-DS tumors. GAPDH was used as a loading control.

Article Snippet: For COL1A1 immunostaining, antigen retrieval was performed in citrate buffer pH 6 in the microwave for 15 minutes after which slides were stained using rabbit-polyclonal anti-COL1A1 antibody (OriGene, Rockville, MD, USA) at a dilution of 1:70.

Techniques: Western Blot

Journal: Neoplasia (New York, N.Y.)

Article Title: Hypoxia Inducible Factors Modify Collagen I Fibers in MDA-MB-231 Triple Negative Breast Cancer Xenografts 1 2

doi: 10.1016/j.neo.2017.11.010

Figure Lengend Snippet: Summary of significant changes observed in the tumors. All comparisons are made with respect to 231-EV tumors

Article Snippet: For COL1A1 immunostaining, antigen retrieval was performed in citrate buffer pH 6 in the microwave for 15 minutes after which slides were stained using rabbit-polyclonal anti-COL1A1 antibody (OriGene, Rockville, MD, USA) at a dilution of 1:70.

Techniques: